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cfx 96 qpcr instrument  (Bio-Rad)


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    Structured Review

    Bio-Rad cfx 96 qpcr instrument
    Cfx 96 Qpcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx+96+qpcr+instrument/cfx96+touch+real+time+pcr+detection+system/pm38703737-133-5-10
    Average 90 stars, based on 1 article reviews
    cfx 96 qpcr instrument - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Transcriptomic analysis and competing endogenous RNA network in the human endometrium between proliferative and mid-secretory phases
    Article Snippet: .. To determine mRNA and lncRNA expression levels, cDNA was synthesized using M-MLV reverse transcriptase (Promega). qPCR was performed using a CFX 96 qPCR instrument and iQ SYBR Green Supermix (Bio-Rad Laboratories) with the following amplification conditions: Initial denaturation at 95 ̊C for 3 min; followed by 40 cycles of denaturation at 95 ̊C for 10 s, 60 ̊C for 10 s, and extension at 72 ̊C for 15 s. We performed TaqMan microRNA assays for determination of miRNA expression levels. ..

    Synthesized:

    Article Title: Transcriptomic analysis and competing endogenous RNA network in the human endometrium between proliferative and mid-secretory phases
    Article Snippet: .. To determine mRNA and lncRNA expression levels, cDNA was synthesized using M-MLV reverse transcriptase (Promega). qPCR was performed using a CFX 96 qPCR instrument and iQ SYBR Green Supermix (Bio-Rad Laboratories) with the following amplification conditions: Initial denaturation at 95 ̊C for 3 min; followed by 40 cycles of denaturation at 95 ̊C for 10 s, 60 ̊C for 10 s, and extension at 72 ̊C for 15 s. We performed TaqMan microRNA assays for determination of miRNA expression levels. ..

    Reverse Transcription:

    Article Title: Transcriptomic analysis and competing endogenous RNA network in the human endometrium between proliferative and mid-secretory phases
    Article Snippet: .. To determine mRNA and lncRNA expression levels, cDNA was synthesized using M-MLV reverse transcriptase (Promega). qPCR was performed using a CFX 96 qPCR instrument and iQ SYBR Green Supermix (Bio-Rad Laboratories) with the following amplification conditions: Initial denaturation at 95 ̊C for 3 min; followed by 40 cycles of denaturation at 95 ̊C for 10 s, 60 ̊C for 10 s, and extension at 72 ̊C for 15 s. We performed TaqMan microRNA assays for determination of miRNA expression levels. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptomic analysis and competing endogenous RNA network in the human endometrium between proliferative and mid-secretory phases
    Article Snippet: .. To determine mRNA and lncRNA expression levels, cDNA was synthesized using M-MLV reverse transcriptase (Promega). qPCR was performed using a CFX 96 qPCR instrument and iQ SYBR Green Supermix (Bio-Rad Laboratories) with the following amplification conditions: Initial denaturation at 95 ̊C for 3 min; followed by 40 cycles of denaturation at 95 ̊C for 10 s, 60 ̊C for 10 s, and extension at 72 ̊C for 15 s. We performed TaqMan microRNA assays for determination of miRNA expression levels. ..

    Article Title: A Nematode Calreticulin, Rs-CRT, Is a Key Effector in Reproduction and Pathogenicity of Radopholus similis
    Article Snippet: .. The primers Actin-F/Actin-R ( ) were employed to amplify β-actin as a reference gene [ ]. qPCR was performed using a CFX-96 qPCR instrument (Bio-Rad) with iTaq Universal SYBR Green Supermix (Bio-Rad). .. The initial data analysis was carried out using Bio-Rad CFX-96 manager software, which created Ct values and extrapolated the relative levels of PCR products from standard curves.

    Article Title: Identification of a proteolysis regulator for an essential enzyme in Mycobacterium tuberculosis
    Article Snippet: .. Reactions were monitored using a BioRad CFX 96 qPCR instrument equipped with a FRET channel. .. Homologs of CoaX Mtb were retrieved from sequence profiles searches using the CoaX Mtb amino acid sequence as query in a PSI-BLAST search ( ) against the non-redundant protein database from National Center for Biotechnology Information (NCBI) that was clustered down to 50% identity (nr50).

    SYBR Green Assay:

    Article Title: Transcriptomic analysis and competing endogenous RNA network in the human endometrium between proliferative and mid-secretory phases
    Article Snippet: .. To determine mRNA and lncRNA expression levels, cDNA was synthesized using M-MLV reverse transcriptase (Promega). qPCR was performed using a CFX 96 qPCR instrument and iQ SYBR Green Supermix (Bio-Rad Laboratories) with the following amplification conditions: Initial denaturation at 95 ̊C for 3 min; followed by 40 cycles of denaturation at 95 ̊C for 10 s, 60 ̊C for 10 s, and extension at 72 ̊C for 15 s. We performed TaqMan microRNA assays for determination of miRNA expression levels. ..

    Article Title: A Nematode Calreticulin, Rs-CRT, Is a Key Effector in Reproduction and Pathogenicity of Radopholus similis
    Article Snippet: .. The primers Actin-F/Actin-R ( ) were employed to amplify β-actin as a reference gene [ ]. qPCR was performed using a CFX-96 qPCR instrument (Bio-Rad) with iTaq Universal SYBR Green Supermix (Bio-Rad). .. The initial data analysis was carried out using Bio-Rad CFX-96 manager software, which created Ct values and extrapolated the relative levels of PCR products from standard curves.

    Amplification:

    Article Title: Transcriptomic analysis and competing endogenous RNA network in the human endometrium between proliferative and mid-secretory phases
    Article Snippet: .. To determine mRNA and lncRNA expression levels, cDNA was synthesized using M-MLV reverse transcriptase (Promega). qPCR was performed using a CFX 96 qPCR instrument and iQ SYBR Green Supermix (Bio-Rad Laboratories) with the following amplification conditions: Initial denaturation at 95 ̊C for 3 min; followed by 40 cycles of denaturation at 95 ̊C for 10 s, 60 ̊C for 10 s, and extension at 72 ̊C for 15 s. We performed TaqMan microRNA assays for determination of miRNA expression levels. ..

    Article Title: In vitro and in vivo activities of a trithiolato-diRuthenium complex conjugated with sulfadoxine against the apicomplexan parasite Toxoplasma gondii.
    Article Snippet: DNA concentrations were adjusted to 5 ng/μl, and qPCR reactions were performed in a final volume of 10 μl containing 1x SensiFast master mix (Bioline, Meridian Bioscience), 0.5 μM of reverse and forward primers, 0.1 μM of 529rpeQ-P probe, 0.3 mM dUTP, and one unit of heat-labile Uracil DNA Glycosylase (UDG) (Hänggeli et al., 2022). .. Amplification was performed in the Bio-Rad CFX 96 QPCR instRUment (Biorad) using the following thermal profile: (1) initial incubation of 10 min at 42 ◦C, followed by (2) denaturation step of 5 min at 95 ◦C and (3) 50 cycles of two-step amplification (10 s at 95 ◦C and 20 s at 62 ◦C). ..

    Article Title: Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in Toxoplasma gondii by CRISPR-Cas9.
    Article Snippet: PCR amplification was performed in a total reaction mixture of 10 μL containing 1x SensiFast master mix (Bioline, Meridian Bioscience), 0.5 μM of reverse and forward primers, 0.1 μM of DHFRQ-P probe, 0.3 mM dUTP, and one unit of heat-labile Uracil DNA Glycosylase (UDG) [56]. .. A Bio-Rad CFX 96 QPCR instrument (Biorad) was used with the following thermal profile: (1) initial incubation of 10 min at 42 ̊C, followed by (2) denaturation step of 5min at 95 ̊C and (3) 50 cycles of two-step amplification (10 s at 95 ̊C and 20 s at 62 ̊C). ..

    other:

    Article Title: Comparative Proteomic Analysis of Toxoplasma gondii Rh Wild-type and Four SRS29B (SAG1) Knock-Out Clones Reveals Significant Differences Between Individual Strains
    Article Snippet: Total RNA was extracted from tachyzoites using the NucleoSpin RNA isolation kit (MachereyNagel, Duren, Germany).

    Incubation:

    Article Title: In vitro and in vivo activities of a trithiolato-diRuthenium complex conjugated with sulfadoxine against the apicomplexan parasite Toxoplasma gondii.
    Article Snippet: DNA concentrations were adjusted to 5 ng/μl, and qPCR reactions were performed in a final volume of 10 μl containing 1x SensiFast master mix (Bioline, Meridian Bioscience), 0.5 μM of reverse and forward primers, 0.1 μM of 529rpeQ-P probe, 0.3 mM dUTP, and one unit of heat-labile Uracil DNA Glycosylase (UDG) (Hänggeli et al., 2022). .. Amplification was performed in the Bio-Rad CFX 96 QPCR instRUment (Biorad) using the following thermal profile: (1) initial incubation of 10 min at 42 ◦C, followed by (2) denaturation step of 5 min at 95 ◦C and (3) 50 cycles of two-step amplification (10 s at 95 ◦C and 20 s at 62 ◦C). ..

    Article Title: Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in Toxoplasma gondii by CRISPR-Cas9.
    Article Snippet: PCR amplification was performed in a total reaction mixture of 10 μL containing 1x SensiFast master mix (Bioline, Meridian Bioscience), 0.5 μM of reverse and forward primers, 0.1 μM of DHFRQ-P probe, 0.3 mM dUTP, and one unit of heat-labile Uracil DNA Glycosylase (UDG) [56]. .. A Bio-Rad CFX 96 QPCR instrument (Biorad) was used with the following thermal profile: (1) initial incubation of 10 min at 42 ̊C, followed by (2) denaturation step of 5min at 95 ̊C and (3) 50 cycles of two-step amplification (10 s at 95 ̊C and 20 s at 62 ̊C). ..



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